Nivaar worked solution (AI-drafted; not reviewed by a licensed engineer)
National Exams — December 2018 — 04-BS-13, Biology. Three-hour, closed-book exam (one double-sided aid sheet permitted, approved Casio/Sharp calculator allowed). Format: Part I offers 5 questions (any 3 constitute a complete answer, 20 marks each) and Part II offers 3 questions (any 2 constitute a complete answer, 20 marks each) — a full paper is 5 questions. All 8 numbered questions are solved below for completeness (renumbered Q1–Q8 continuously: Q1–Q5 = Part I, Q6–Q8 = Part II). Q1, Q2, Q3, and Q4 are calculation/stoichiometry questions; Q5, Q6, Q7, and Q8 are essay questions.
Reference texts: Shuler & Kargi, Bioprocess Engineering: Basic Concepts (2nd ed., Prentice Hall) — elemental/electron balances, yield coefficients, maintenance-associated product formation, fermenter mass and energy balances; Madigan et al., Brock Biology of Microorganisms (15th ed., Pearson) — bacterial nutrition, transport mechanisms, cell-wall structure, pure-culture technique, sterilization methods; Toledo, Fundamentals of Food Process Engineering (3rd ed., Springer) — plant/animal tissue rheology and gross structure.