Paper format: National Exams, December 2015 — 04-Bio-B10 Analytical Biochemistry. Three hours, closed book, any non-communicating calculator. Six questions of equal value (20 marks each); five constitute a complete paper and only the first five appearing in the answer book are marked. All six are solved here, because this set is a study resource rather than an examination script. Every question is essay/descriptive (technique principle, interpretation of an instrument trace or image), with no numerical calculation on this sitting.
Reference texts (the books a candidate should have reviewed for this subject):
- J. Sambrook & D. W. Russell, Molecular Cloning: A Laboratory Manual, 3rd ed. — chs. on protein/native and SDS electrophoresis, PCR and real-time PCR, and affinity purification of antibodies and recombinant proteins.
- K. Wilson & J. Walker (eds.), Principles and Techniques of Biochemistry and Molecular Biology, 8th ed. — chs. on electrophoretic techniques, PCR, flow cytometry, and affinity/lectin chromatography.
- D. A. Skoog, F. J. Holler & S. R. Crouch, Principles of Instrumental Analysis, 7th ed. — chs. on infrared/FTIR spectroscopy (Michelson interferometer) and scanning-probe (atomic force) microscopy.
- B. Alberts et al., Molecular Biology of the Cell, 6th ed. — supporting background on fluorescent-protein reporters, antibody structure, and inducible gene expression.
