20-Bio-B10 Biomechanical Device Design & Human Factors: December 2017

Paper format: National Exams, December 2017 — 04-Bio-B10 Analytical Biochemistry. Three hours, closed book, any non-communicating Casio/Sharp calculator. Six questions of equal value (20 marks each); five constitute a complete paper and only the first five appearing in the answer book are marked. All six are solved here, because this set is a study resource rather than an examination script. The paper is essay/descriptive throughout, with two embedded PCR copy-number sub-questions (Q2b, Q2c) that carry numeric content.

Reference texts (the books a candidate should have reviewed for this subject):

• J. Sambrook & D. W. Russell, Molecular Cloning: A Laboratory Manual, 3rd ed. — chs. on protein/native and SDS electrophoresis, PCR, and chromatographic purification.
• K. Wilson & J. Walker (eds.), Principles and Techniques of Biochemistry and Molecular Biology, 8th ed. — chs. on electrophoretic techniques, PCR, flow cytometry, and ion-exchange/gel-permeation chromatography.
• D. A. Skoog, F. J. Holler & S. R. Crouch, Principles of Instrumental Analysis, 7th ed. — chs. on FTIR spectroscopy (Michelson interferometer, Fourier Transform) and NMR spectroscopy.
• B. Alberts et al., Molecular Biology of the Cell, 6th ed. — supporting background on antibody structure and flow-cytometric analysis of cell-surface receptors.

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