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04-BS-13 · May 2013

Question 3 of 10: Energy Balance on a Continuous Fermenter

Nivaar worked solution (AI-drafted; not reviewed by a licensed engineer)

Notes on this paper

National Exams — May 2013 — 04-BS-13, Biology. Three-hour, closed-book exam (one double-sided aid sheet permitted, approved Casio/Sharp calculator allowed). Format: Part I offers 6 questions (any 3 constitute a complete answer, 20 marks each) and Part II offers 4 questions (any 2 constitute a complete answer, 20 marks each) — a full paper is 5 questions. All 10 are solved below for completeness. Most questions require an essay-format answer; Q1–Q4 are calculation questions.

Reference texts: Shuler & Kargi, Bioprocess Engineering: Basic Concepts (2nd ed., Prentice Hall) — elemental/electron balances, yield coefficients, fermenter energy balances; Madigan et al., Brock Biology of Microorganisms (15th ed., Pearson) — bacterial/viral/fungal morphology and physiology; Toledo, Fundamentals of Food Process Engineering (3rd ed., Springer) — plant/animal tissue structure, rheology, water activity.

Question 3: Energy Balance on a Continuous Fermenter (20 marks)

Question text not reproduced: the examination questions are © Engineers and Geoscientists BC. Open the official past paper (linked at the top of this page) to read the question, then follow the worked solution below.

Given. Metabolic heat-generation rate $q=27.6\ \text{kJ}/(\text{kg}\!\cdot\!\text{h})$; cell mass held up in fermenter $M=2$ kg; feed temperature $T_{\text{feed}}=25\,{}^{\circ}\text{C}$; mass flow rate $\dot m=1.025$ kg/h; specific heat $c_p=4\ \text{kJ}/(\text{kg}\!\cdot\!{}^{\circ}\text{C})$; growth ceiling $T_{\max}=42\,{}^{\circ}\text{C}$; fermenter well-insulated (no external heat loss); steady state (exit temperature = fermenter temperature, constant with time).

Find. The steady-state fermenter (exit) temperature, and whether the cells survive.

Approach. At steady state with an insulated vessel, all metabolic heat generated by the cell mass must leave as sensible heat carried by the flow between feed and exit temperature — a single algebraic energy balance gives the exit temperature directly.

  1. Total heat generation rate. $$\dot Q_{\text{gen}}=q\cdot M = 27.6\left(\frac{\text{kJ}}{\text{kg}\!\cdot\!\text{h}}\right)(2\ \text{kg}) = 55.2\ \text{kJ/h}.$$
  2. Steady-state energy balance. Well-insulated $\Rightarrow$ no heat loss to the surroundings, so all generated heat raises the stream temperature from feed to exit: $$\dot Q_{\text{gen}} = \dot m\,c_p\,(T_{\text{exit}}-T_{\text{feed}}).$$
  3. Solve for the temperature rise. $$\Delta T = \frac{\dot Q_{\text{gen}}}{\dot m\,c_p} = \frac{55.2}{(1.025)(4)} = \frac{55.2}{4.1} = 13.46\,{}^{\circ}\text{C}.$$
  4. Exit (fermenter) temperature. $$T_{\text{exit}} = 25 + 13.46 = \boxed{38.46\,{}^{\circ}\text{C}}.$$ Since $38.46\,{}^{\circ}\text{C} < 42\,{}^{\circ}\text{C}$, the fermenter operates below the growth ceiling and the cells will survive.
QuantityResult
Total metabolic heat rate55.2 kJ/h
Temperature rise, feed→exit13.46 °C
Steady-state fermenter temperature38.46 °C
Cells survive? (limit 42 °C)Yes — 3.54 °C of margin