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04-BS-13: May 2016

Nivaar worked solution (AI-drafted; not reviewed by a licensed engineer)

  1. Question 1 Plant vs. Animal Cell Structure and the Plant Cell Wall
  2. Question 2 Fed-Batch Fermenter — Total and Substrate Mass Balances
  3. Question 3 Septic Tank Unsteady-State Organic-Material Balance
  4. Question 4 Growth Stoichiometry and Yield Coefficients on Glucose
  5. Question 5 Ammonia and Oxygen Demand for Recombinant Protein Production
  6. Question 6 Cooling Requirement for Immobilized-Cell Glutamic Acid Production
  7. Question 7 Rapid Methods, MPN, Coliform Plate Count, and Virus Structure
  8. Question 8 Hybridoma Batch Culture — Specific Growth Rate and Doubling Time
  9. Question 9 Growth Yields, Microbial Classification, Monod Kinetics, and Thermal Death

Start with Question 1 →

National Exams — May 2016 — 04-BS-13, Biology. Three-hour, closed-book exam (one double-sided aid sheet permitted, approved calculator allowed). Format: Part I offers 5 questions (any 3 constitute a complete answer, 20 marks each; Q2 itself offers two alternative sub-problems) and Part II offers 3 questions (any 2 constitute a complete answer, 20 marks each) — a full paper is 5 questions. All 8 numbered questions (with both alternatives of Q2) are solved below for completeness. Q2–Q7 are calculation/derivation questions; Q1, Q6(a)(b)(d), and Q8 are essay questions.

Reference texts: Shuler & Kargi, Bioprocess Engineering: Basic Concepts (2nd ed., Prentice Hall) — elemental/electron balances, yield coefficients, fermenter mass and energy balances, growth kinetics; Madigan et al., Brock Biology of Microorganisms (15th ed., Pearson) — bacterial/viral structure, rapid methods, MPN; Toledo, Fundamentals of Food Process Engineering (3rd ed., Springer) — plant tissue structure and mechanical properties.