23-Chem-B4 Biochemical Engineering · December 2016
Nivaar worked solution (AI-drafted; not reviewed by a licensed engineer)
National Exam 04-Chem-B4, Biochemical Engineering — December 2016. 3 hours, Closed-Book Exam (one approved Casio or Sharp calculator model permitted). Per the exam notes, FIVE (5) questions constitute a complete paper and all five must be answered; most require a short-essay-format answer.
Reference texts: Shuler & Kargi, Bioprocess Engineering: Basic Concepts, 2nd ed.; Bailey & Ollis, Biochemical Engineering Fundamentals, 2nd ed.; Madigan et al., Brock Biology of Microorganisms, 13th ed.
Question text not reproduced: the examination questions are © Engineers and Geoscientists BC. Open the official past paper (linked at the top of this page) to read the question, then follow the worked solution below.
Continuous HTST sterilization heats a flowing medium rapidly to a high temperature, holds it briefly in a holding tube (killing spores while minimizing nutrient degradation, since destruction of heat-resistant spores has a higher activation energy than destruction of heat-labile nutrients — so a short time at high temperature kills spores while sparing more nutrients than a long time at low temperature), then cools it quickly. The two standard ways of delivering that heat are direct steam injection and indirect heating through a wall, most efficiently arranged with heat regeneration.
High-pressure culinary steam is injected directly into the product stream through a steam injector/infusion head, mixing and heating it to sterilization temperature almost instantaneously (heat-up times of a fraction of a second, since there is no wall to conduct through). After the holding tube, the product is flash-cooled under vacuum, which simultaneously removes the water added by the condensing steam and drops the temperature rapidly by flash evaporation.
The product is heated indirectly across a metal wall (plate or tubular heat exchanger), first by regenerative exchange with the already-sterilized outgoing product (recovering a large fraction of the heat that would otherwise be wasted), then finished to sterilization temperature by a steam-jacketed final heater. After holding, the hot sterile product gives its heat back to the incoming cold feed across the same regenerative exchanger before final cooling.
| Feature | Direct steam injection | Indirect (regenerative wall heating) |
|---|---|---|
| Heat-up rate | Extremely fast (no wall resistance) | Slower (limited by wall heat-transfer coefficient and fouling) |
| Product dilution | Yes — steam condensate adds water, must be removed by vacuum flash-cooling | None — product never contacts the heating medium |
| Energy efficiency | Lower — no heat regeneration; all latent heat of the injected steam is a fresh cost | Higher — regenerative section can recover up to ~90% of the heat |
| Fouling / burn-on risk | Low — brief contact, no hot solid surface | Higher — viscous/particulate product can foul the hot exchanger wall, raising cleaning (CIP) frequency |
| Best suited to | Thin, low-viscosity liquids needing the shortest possible thermal exposure | Large continuous throughput where energy recovery and no dilution matter most |