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20-Bio-A5 Systems Analysis & Control: December 2017

Nivaar worked solution (AI-drafted; not reviewed by a licensed engineer)

  1. Question 1 Quasi-Steady-State Rate Law for a Two-Site Enzyme Mechanism
  2. Question 2 Determining the Inhibition Constant and Inhibition Type
  3. Question 3 Immobilized-Enzyme Batch Reactor with Enzyme Deactivation
  4. Question 4 Elemental-Balance Stoichiometry of Aerobic Glucose-to-Biomass Growth
  5. Question 5 Continuous Fermentation with Cell Recycle (Self-Flocculating Yeast)
  6. Question 6 Batch, Chemostat, and Fed-Batch Culture Kinetics Compared

Start with Question 1 →

National Exams / EGBC — December 2017 — 04-Bio-A5 Enzyme and Microbial Kinetics. Three-hour open-book examination; any non-communicating calculator is permitted. Six questions constitute a complete paper. Content spans quasi-steady-state derivation of a two-site enzyme mechanism, inhibition kinetics fitted from experimental rate data, immobilized-enzyme deactivation in a batch reactor, elemental-balance stoichiometry of aerobic biomass growth, a continuous fermentation with cell recycle, and a batch/chemostat/fed-batch culture-kinetics comparison.

Reference texts: Bailey & Ollis, Biochemical Engineering Fundamentals (2nd ed.) — enzyme kinetics and inhibition, immobilized-enzyme deactivation, stoichiometry of microbial growth, continuous culture with cell recycle, batch/chemostat/fed-batch kinetics. All quantities are used exactly as printed on the exam.

Check: the printed marking scheme (page 1) lists mark weights for Questions 1–5 only (10 + 15 + 15 + 20 + 25 = 85 marks), even though instruction 3 states the paper has SIX questions and Question 6 (parts a–d) is fully printed on pages 4–5. We adopt 15 marks for Question 6 — matching Question 3's single-part weight — so the paper totals a clean 100 marks; this is the same class of front-page marking-scheme typo already documented elsewhere in this discipline, not a content gap.